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addgene 105553 aav1 penn aav hsyn cre wpre hgh  (Addgene inc)


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    Addgene inc addgene 105553 aav1 penn aav hsyn cre wpre hgh
    Addgene 105553 Aav1 Penn Aav Hsyn Cre Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav1+aav+cre+addgene/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__01__14__699532-136-5-5
    Average 96 stars, based on 311 article reviews
    addgene 105553 aav1 penn aav hsyn cre wpre hgh - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Bioprocessing:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Software:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Imaging:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Microscopy:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Real-time Polymerase Chain Reaction:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Blocking Assay:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..

    Membrane:

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 Article ll OPEN ACCESS ..

    Article Title: α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GibcoTM Neurobasal A medium Thermo Fisher Scientific Cat #12349-015 HBSS Thermo Fisher Scientific Cat #14175-095 Laminin Roche Cat #11243217001 B27 supplement (50X) Thermo Fisher Scientific Cat #17504044 Fetal bovine serum Thermo Fisher Scientific Cat #26140079 Sodium bicarbonate Thermo Fisher Scientific Cat #S233500 Sodium chloride Thermo Fisher Scientific Cat #S271-3 Magnesium sulfate Sigma-Aldrich Cat #M7506 Calcium chloride MP Biomedicals Cat #153502 Potassium phosphate dibasic Sigma-Aldrich Cat #P3786 0.9% Sodium chloride Aqualite System Cat #0409-7138-09 Insulin (Humulin R) Thermo Fisher Scientific Cat #NC1415864 Isoprenaline hydrochloride Abcam Cat #ab146724 Gabapentin Sigma-Aldrich Cat #PHR1049 Adenosine Sigma-Aldrich Cat #A4036 Tris-HCl Sigma-Aldrich Cat #10812846001 Sodium deoxycholate Sigma-Aldrich Cat #D6750 Np-40 (IGEPAL CA-630) Thermo Fisher Scientific Cat #J61055 Sodium dodecyl sulfate (SDS) Bio-Rad Cat #1610302 0.5 M Ethelene-di-amine-tetraacetic acid (EDTA) Thermo Fisher Scientific Cat #AM9260G Ponceau S solution Sigma-Aldrich Cat #P7170 Blotting grade blocker non-fat dry milk Bio-Rad Cat #1706404XTU cOmpleteTM, EDTA-free protease inhibitor cocktail Sigma-Aldrich Cat #04693132001 PhosSTOPTM Sigma-Aldrich Cat #4906845001 Collagenase, type 1 Worthington Cat # LS004194 Calcitonin gene-related peptide rat Sigma-Aldrich Cat #C0292 Fluoro-Gold Fluorochrome, LLC Cat #Fluoro-Gold 20mg Cytofix/cytoperm BD Biosciences Cat #51-2090KZ Perm wash BD Biosciences Cat #51-2091KZ Brilliant stain buffer BD Biosciences Cat #566349 Fixable viability, eFlour780 eBioscience Cat #65-0865 Critical commercial assays Catecholamines ELISA Kit Abcam Cat #ab287795 Hematoxylin and eosin stain kit Abcam Cat #ab245880 Glycerol assay kit (cell-based) Abcam Cat #ab133130 Free fatty acid quantification kit Sigma-Aldrich Cat #MAK044 Mouse CGRP ELISA kit (colorimetric) Novus Cat #NBP3-00522 Amylase assay kit (colorimetric) Abcam Cat #ab102523 Lipase assay kit (colorimetric) Abcam Cat #ab102524 Protein assay dye Biorad Cat #5000006 PierceTM BCA protein assay kit Thermo Fisher Scientific Cat #23225 SuperScript VILO cDNA synthesis kit Thermo Fisher Scientific Cat #11574050 Fast SYBR green master mix Applied Biosystems Cat #4385612 Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory Cat #000664; RRID: IMSR_JAX:000664 Ai9 (RCL-tdTomato) The Jackson Laboratory Cat #007909; RRID: IMSR_JAX:007909 (Continued on next page) e2 Cell Reports Medicine 5, 101525, May 21, 2024 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Cacna2d1fl/fl The Jackson Laboratory Cat #030704; RRID: IMSR_JAX:030704 Nav1.8-Cre The Jackson Laboratory Cat #:036564; RRID: IMSR_JAX:036564 Oligonucleotides See Table S2 for primer sequences N/A Recombinant DNA AAV-tdTomato Addgene Cat #105545-AAV1 AAV-eGFP Addgene Cat #105530-AAV1 AAV-Cre Addgene Cat #105537-AAV1 AAV-Cre-eGFP Addgene Cat #105545-AAV1 Software and algorithms NIS-Elements imaging software – version 5.11.00 Nikon https://www.microscope.healthcare. nikon.com/products/software/nis-elements FIJI (ImageJ) NIH https://imagej.net/software/fiji/ GraphPad Prism - version 9.5.1 GraphPad https://www.graphpad.com SoftMax PRO 7.1 Molecular Devices https://moleculardevices.com Gen 3.10 Agilent BioTEK https://www.agilent.com/en/support/ biotek-software-releases Cytoscape version 3.10.1 Open source cytoscape.org STRING version 12.0 Open source https://string-db.org/ Other FisherbrandTM SuperfrostTM plus microscope slide Fisher Scientific Cat #22-037-246 StepOnePlusTM Real-Time PCR system Thermo Fisher Scientific Cat #4376600 BIOTEK Synergy H1 microplate reader Agilent BioTEK Cat #11-120-535 Spectra MAX190 microplate reader Molecular Devices N/A 24-Well Plate (Black) Sigma Aldrich Cat #CLS3925 Lysing Matrix D (RNAse-DNAse Free) MP Biomedicals Cat #116913050-CF Bullet Blender Homogenizer Next Advance Cat #BBX24 Sonicator, model 120 Fisher Scientific Cat #FB120110 Heating block Fisher Scientific Cat #88860021 Isotemp oven (model 516G) Fisher Scientific Cat #13246516G Cell strainer, 500 mm Fisher Scientific Cat #NC0822591 Cell strainer, 70 mm Fisher Scientific Cat #22-363-548 Chambered coverslip (8 wells) Ibidi Cat #80806 PVDF membrane, 0.2 mm Bio-Rad Cat #1620177 Nitrocellulose membrane, 0.2 mm Bio-Rad Cat #1620112 OPEN ACCESS ..



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